Optimization of carboxymetyhlcellulase and polyoses production from oil palm empty fruit bunch fermentation by Trichoderma Virens

Abstract

Optimization of CMCase and polyoses production by Trichoderma virens via batch culture fermentation of pretreated oil palm empty fruit bunch (OPEFB) was carried out using statistical software of Design Expert ® version 6.0.4. The General Factorial Design was applied to study the influence of nitrogen sources towards CMCase production. Combination of peptone and ammonium sulphate with C:N 39.2 mM significantly increased CMCase activities, specific activity and total polyoses production. Mixture of peptone and ammonium sulphate was further utilized for screening of significant factors for CMCase production using 2-Level Factorial Design. The significant factors that influenced CMCase production were temperature, pH, fermentation time, concentration of substrate and Tween 80. These significant factors were used for optimization process using central composite design (CCD). The optimal conditions which stimulate the highest CMCase production and its specific activity were 7 days fermentation, 0.4% (v/v) of Tween 80 concentration, 0.1% (w/v) OPEFB concentration, 25oC and initial pH at 5.56. Under those optimum conditions, CMCase production was 0.39 U/mL and specific activity at 0.24 U/mg, indicated the improvement of 1.9 fold as compared to that of non optimized condition. The polyoses production increased for 2.5 fold in comparison with that of non optimized conditions. Various types of polyoses were produced such as cellobiose, maltose, glucose, xylose, galactose, arabinose and mannose.