Chemical constituents from the leaves of Garcinia Parvifolia

Abstract

The chemical constituents of the leaves of G. parvifolia (known as kandis) have been investigated. Extraction of dried leaves was successfully done by cold extraction method using ethanol as solvent, followed by partitioning using n-hexane, chloroform and ethyl acetate. Fractionation and purification on the n-hexane crude extract by using Vacuum Liquid Chromatography (VLC) and Column Chromatography (CC) have resulted four compounds. The elucidation of the structures were carried out by spectroscopic techniques using IR, 1H, 13C, DEPT and GC-MS. Analysis by spectroscopic data showed the isolated compounds are ß- sitosterol, squalene, friedelin-3ß-ol, and mixture of friedelin and friedelin-3ß-ol. The antibacterial activity of crude extracts was carried out using disc diffusion methods with eight strains of bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Pseudomonas putida. Antibacterial screening showed that the chloroform crude extract gave a strong towards Klebsiella pneumonia, Pseudomonas aeruginosa and Pseudomonas putida with minimum inhibition concentration of 450 µg/mL