Cyclic voltammetric study of aflatoxin G1 at the mercury electrode

Abstract

A cyclic voltammetry (CV) study of aflatoxin G1 (AFG1) in Britton-Robinson buffer (BRB) using a control growth mercury electrode (CGME) is described. CV was carried out by anodic and cathodic potential scan over the range of 0 to -1500 mV with no accumulation time. The effect of the different scan rates and pH of BRB on the peak height and peak potential of the analyte were also studied. The results from this study showed that the reduction process on the hanging mercury electrode gives a single characteristic cathodic peak at -1184 to -1246 mV ( versus Ag/AgCl ) in BRB pH of 3.0 to 10.0. The BRB pH of 9.0 was noted as the best condition for the detection of AFG1 as the peak gives a maximum peak current. Effect of the scan rate and pH of BRB on both responses has shown that the reduction of AFG1 is irreversible, pH dependent and the limiting current is adsorption controlled.