Jing, Ng Hong and Ab. Wahab, Roswanira and Mohd. Taha, Aishah and Abdul Rashid, Noor Aini and Huyop, Fahrul (2009) A further characterization of 3-chloropropionic acid dehalogenase from rhodococcus sp. HJ1. In: International Congress of Malaysian Society for Microbiology (ICMSM 2009), 2009, Park Royal Hotel, Pulau Pinang.
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Official URL: http://dx.doi.org/10.3923/jm.2008.482.488
Abstract
The main aim of the present study is to further characterize a new dehalogenase enzyme found in the crude extracts from Rhodococcus sp. The ability of the enzyme to catalyze the dehalogenation of various halogen-substituted organic acids was investigated and the highest activity was found with 3-chloropropionic acid as a sole carbon source in the growth medium. The enzyme followed Michaelis-Menten kinetics and the Km for 3-chloropropionic acid was 0.2 mM. Maximum activity was found at pH 7.6 at 30°C. The enzyme activity in the cell-free extract was unaffected by diaminoethane tetraacetic acid (EDTA), dithiothreitol (DTT) or by Mn and Zn ions but was reduced by HgCl2 (70%) and Pb(NO3)2 (80%). The enzyme removed the chlorine atom present on a number of 3- and 4-carbon alkanoic acids if the halogen was on the β-position.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Uncontrolled Keywords: | organic acid, sole carbon source, Michaelis-Menten kinetics |
| Subjects: | Q Science > Q Science (General) T Technology > TA Engineering (General). Civil engineering (General) |
| Divisions: | Biosciences and Bioengineering |
| ID Code: | 14665 |
| Deposited By: | Narimah Nawil |
| Deposited On: | 12 Sep 2011 02:12 |
| Last Modified: | 15 Jun 2020 03:46 |
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