Inhibitory effects of swietenia mahagoni seeds extract on A-glucosidase and A-amylase

Abstract

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays. Swietenia mahagoni seeds were extracted by using supercritical carbon dioxide (SC-CO2) extraction at pressures of 20-30 MPa and temperatures of 40-60°C. The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays. All data obtained were expressed as mean ± standard deviation for triplicate experiments. One way analysis was used for statistical significance by using statistica software version 7.0 (StartSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software. Swietenia mahagoni seeds extract have a strong inhibition of α-glucosidase enzyme activity (98.4% ± 0.2) but a moderate inhibition of α- amylase enzyme activity (34.9% ± 1.2). These findings implied that Swietenia mahagoni seeds extract could be an effective natural antidiabetic agent.