Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius

Abstract

Trigonelline was a plant hormone that is built up in a plant from the methylation of the nitrogen atom of niacin. It was responsible for induction of G2 phase arrest in the root apices of many plant species. Trigonelline was also capable of reducing the blood sugar level and thus treating diabetes type II. In this study, effect of methyl jasmonate, MeJa (50 µM, 100 µM and 200 µM) on trigonelline content in suspension cell culture of Abrus precatorius using High Performance –Liquid Chromatography for trigonelline was investigated. The effect of different inoculum size (0.1 g and 0.5 g) on cell biomass and trigonelline content of suspension cell culture of A.precatorius was studied. Suspension cell culture of A. precatorius was developed in a 100 mL conical flask containing 30 mL MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L 2, 4- D. Results showed that 0.5 g inoculum had higher cell biomass with maximum biomass of 0.0795 g than 0.1 g inoculum that had biomass decrement throughout the culture. Furthermore, addition of 50 µM MeJa had multiplied trigonelline content to five times (6.62 mg/L) compared to the control (1.41 mg/L) for a period of 2 weeks. The correlation coefficient test indicated that cell biomass was not correlated with trigonelline content. Different increment of cell biomass with trigonelline suggested that trigonelline was not involved in regulating cell cycle of A. precatorius. In conclusion, 0.5 g inoculum size was a better size compared to 0.1 g inoculum suspension cell culture. The optimum trigonelline during cell growth of A.precatorius was also discovered.