Cytostatic activity of clinoptilolite against human cervical cancer cell lines using three different media-sterilization techniques

Abstract

Clinoptilolite has been proven useful in cancer therapy in the l ast decade . The use of natural zeolite such as clinoptilolite requires some purification of the raw material before it can be applied in biomedical applications, yet microorganism contamin ation is still viewed as a possible issue for most researchers . T her efore, the clinoptilolite - treated media must under go filter sterilization process prior to its use in any in vitro research of human cell studies . The aim of this study was to determine whether clinoptilolite cytostatic effect on cervical cancer (HeLa) cel ls will differ when a different media - sterilization technique is used on the clinoptilolite - treated medium. Comparison of the effect of clinoptilolite - treated medium on cell cytostatic activity was made between three sterilization methods; heat sterilizati on using an autoclave , UV radiation and filter sterilization. Results showed that there were inhibition of HeLa cell growth after clinoptilolite treatment in all three media - sterilization techniques. Significant differences were shown in all except between sterilizing using UV radiation and filter sterilization. This proved that in vitro cytostatic activity of clinoptilolite on human cervical cancer cells may be affected if different sterilization methods are used to sterilize the pre - treated media. Nonethe less , filter sterilization is still the preferred choice for sterilizing treated - media in cell culture.