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Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase

Wong, Fui-Ling and Abdul-Aziz, Azila (2008) Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase. Journal Of Chemical Technology And Biotechnology , 83 . pp. 41-46. ISSN 0268-2575

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Official URL: http://dx.doi.org/10.1002/jctb.1774

Abstract

BACKGROUND: The performances of four types of glucose oxidase (GOD) immobilization materials based on poly(vinyl alcohol) (PVA) were compared. The matrices of interest were chernically-linked PVA, freeze-thawed PVA cryogel, tetramethoxysilane (TMOS) sol-gel-PVA hybrid material, and alumina sol-gel-PVA hybrid material. RESULTS: Overall, the membranes showed good sensitivity except for the chemically cross-linked PVA. However, the main differences with the enzyme immobilization methods were enzyme leakage and values of K-m(app). CONCLUSION: Freeze-thawed PVA-GOD membranes and TMOS-PVA, which showed satisfactory sensitivity and adequate value of K-m(app), were quite promising as support materials for immobilizing GOD. (c) 2007 Society of Chemical Industry.

Item Type:Article
Uncontrolled Keywords:immobilization, glucose oxidase, poly(vinyl alcohol), sol-gel, hybrid
Subjects:Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions:Chemical and Natural Resources Engineering (Formerly known)
ID Code:12517
Deposited By: Liza Porijo
Deposited On:07 Jun 2011 10:14
Last Modified:07 Jun 2011 10:14

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