Universiti Teknologi Malaysia Institutional Repository

Cloning of influenza B NS1 gene in Escherichia Coli

Adel Dawood, Ali (2010) Cloning of influenza B NS1 gene in Escherichia Coli. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering.

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Abstract

The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a.

Item Type:Thesis (Masters)
Additional Information:Thesis (Sarjana Sains (Bioteknologi)) - Universiti Teknologi Malaysia, 2010; Supervisor : Dr. Chan Giek Far
Uncontrolled Keywords:escherichia coli, influenza, NS1B antagonizes
Subjects:R Medicine > RZ Other systems of medicine
Divisions:Biosciences and Bioengineering (Formerly known)
ID Code:12283
Deposited By: Ms Zalinda Shuratman
Deposited On:15 Apr 2011 05:10
Last Modified:27 Jun 2012 00:33

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