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Normal soaking extraction (NSE) of rotenone from Derris elliptica

Zubairi, Saiful Irwan and Sarmidi, M. R. and Aziz, R. A. and Ramli, M. K. A. and Latip, R. and Nordin, N. I. A. (2004) Normal soaking extraction (NSE) of rotenone from Derris elliptica. In: Proceeding of Simposium Kimia Analisis Kebangsaan (SKAM-17), 26-28 August 2004, Swiss Garden Resort, Pahang.



Derris elliptica or the tuba plant contains rotenone, bio-active compound known that has the potential to be used as bio-pesticide. Bio-pesticide is the best-known alternative bio-pesticide which has the potential to replace the use of conventional pesticides, as it is more environmentally friendly. The main objective of the paper is to obtain the best extraction solvent for optimizing the yield in rotenone extraction. Rotenone was extracted from Derris root using three different parts of roots and three types of solvent in Normal Soaking Extraction (NSE). The types of Derris roots are: (1) Fine root (2) Coarse root (3) Stem. The three types of solvent system are: (1) Acetone 95 % (v/v), (2) Chloroform 99.9 % (v/v) and (3) a mixture of ethanol: H2O (9:1) added with oxalic acid (1mg/ml). The liquid crude extracts were further cleaned up to remove the fine debris of roots. The presence of rotenone was confirmed using qualitative analysis Thin Layer Chromatography (TLC) and thereupon the determination of rotenone content was carried out using High Performance Liquid Chromatography (HPLC). From the results obtained, it was found that the Normal Soaking Extraction (NSE) using acetone 95 % (v/v) was the best method to extract the highest yield of rotenone; 1.14 % (w/w).

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:derris elliptica, rotenone, environmentally friendly, normal soaking extraction (NSE)
Subjects:T Technology > TP Chemical technology
Q Science > QH Natural history > QH301 Biology
Divisions:Chemical and Natural Resources Engineering
ID Code:9798
Deposited By: Zalinda Shuratman
Deposited On:25 Mar 2010 04:09
Last Modified:02 Jun 2010 02:03

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