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Metabolomics analysis of herb-partitioned moxibustion treatment on rats with diarrhea-predominant irritable bowel syndrome

Lin, Xianwei and Liu, Xia and Xu, Jingjing and Cheng, Kian-Kai and Cao, Jianan and Liu, Tao and Liu, Qiong and Zhong, Huan and Shen, Guiping and Dong, Jiyang and Chang, Xiaorong (2019) Metabolomics analysis of herb-partitioned moxibustion treatment on rats with diarrhea-predominant irritable bowel syndrome. Chinese Medicine (United Kingdom), 14 (1). pp. 1-10. ISSN 1749-8546


Official URL: http://dx.doi.org/10.1186/s13020-019-0240-2


Background: Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, which is commonly treated with antidiarrhoeal, antispasmodics, serotonergic agents or laxative agents. These treatments provide relief for IBS symptoms but may also lead to undesired side effects. Previously, herb-partitioned moxibustion (HPM) treatment has been demonstrated to be effective in ameliorating symptoms of IBS. However, the underlying mechanism of this beneficial treatment is yet to be established. The aim of the current study was to systematically assess the metabolic alterations in response to diarrhea-predominant IBS (IBS-D) and therapeutic effect of HPM. Methods: Proton nuclear magnetic resonance spectroscopy (1H NMR)-based metabolomics approach was used to investigate fecal and serum metabolome of rat model of IBS-D with and without HPM treatment. Results: The current results showed that IBS-induced metabolic alterations in fecal and serum sample include higher level of threonine and UDP-glucose together with lower levels of aspartate, ornithine, leucine, isoleucine, proline, 2-hydroxy butyrate, valine, lactate, ethanol, arginine, 2-oxoisovalerate and bile acids. These altered metabolites potentially involve in impaired gut secretory immune system and intestinal inflammation, malabsorption of nutrients, and disordered metabolism of bile acids. Notably, the HPM treatment was found able to normalize the Bristol stool forms scale scores, fecal water content, plasma endotoxin level, and a number of IBS-induced metabolic changes. Conclusions: These findings may provide useful insight into the molecular basis of IBS and mechanism of the HPM intervention.

Item Type:Article
Uncontrolled Keywords:Herb-partitioned moxibustion, Irritable bowel syndrome, Metabolomics, Nuclear magnetic resonance
Subjects:Q Science > QD Chemistry
ID Code:96959
Deposited By: Widya Wahid
Deposited On:04 Sep 2022 15:55
Last Modified:04 Sep 2022 15:55

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