Universiti Teknologi Malaysia Institutional Repository

Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives

Ragab, Tamer I. M. and Abd. Malek, Roslinda and Elsehemy, Islam A. and Farag, Mohamed M. S. and Salama, Bassem M. and Abd. EL-Baseer, Mohamed A. and Gamal-Eldeen, Amira M. and El Enshasy, Hesham A. and Esawy, Mona A. (2019) Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives. Journal of Bioscience and Bioengineering, 127 (6). pp. 655-662. ISSN 1389-1723

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1016/j.jbiosc.2018.09.008

Abstract

This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2.0, K2HPO4 3.0 and MgSO4·7H2O 0.2; an increase in both carbohydrates consumption and cell growth depended on increasing the size of the stirred tank bioreactor from 16 L to 150 L. The highest levansucrase production (63.4 U/mL) and levan yield of 47 g/L was obtained after 24 h. Also, the specific levan yield (Yp/x) which reflects the cell productivity increased with the size increase of the stirred tank bioreactor and reached its maximum value of about 29.4 g/g cells. These results suggested that B. subtilis M could play an important role in levan yield on a large scale in the future. Chemical modifications of B. subtilis M crude levan (CL) into sulfated (SL), phosphorylated (PL), and carboxymethylated levans (CML) were done. The difference in CL structure and its derivatives was detected by FT-IR transmission spectrum. The cytotoxicity of CL and its derivatives were evaluated by HepGII, Mcf-7 and CaCo-2. In general most tested levans forms had no significant cytotoxicity effect. In fact, the carboxymethylated and phosphrylated forms had a lower anti-cancer effect than CL. On the other hand, SL had the highest cytotoxicity showing SL had a significant anti-cancer effect. The results of cytotoxicity and cell viability were statistically analyzed using three-way ANOVA.

Item Type:Article
Uncontrolled Keywords:Bacillus subtilis, Chemical modification, Cytotoxicity, Fermentor, Levan
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:96944
Deposited By: Widya Wahid
Deposited On:04 Sep 2022 07:26
Last Modified:04 Sep 2022 07:26

Repository Staff Only: item control page