Ragab, Tamer I. M. and Abd. Malek, Roslinda and Elsehemy, Islam A. and Farag, Mohamed M. S. and Salama, Bassem M. and Abd. EL-Baseer, Mohamed A. and Gamal-Eldeen, Amira M. and El Enshasy, Hesham A. and Esawy, Mona A. (2019) Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives. Journal of Bioscience and Bioengineering, 127 (6). pp. 655-662. ISSN 1389-1723
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Official URL: http://dx.doi.org/10.1016/j.jbiosc.2018.09.008
Abstract
This study focused on kinetics of levan yield by Bacillus subtilis M, in a 150 L stirred tank bioreactor under controlled pH conditions. The optimized production medium was composed of (g/L): commercial sucrose 100.0, yeast extract 2.0, K2HPO4 3.0 and MgSO4·7H2O 0.2; an increase in both carbohydrates consumption and cell growth depended on increasing the size of the stirred tank bioreactor from 16 L to 150 L. The highest levansucrase production (63.4 U/mL) and levan yield of 47 g/L was obtained after 24 h. Also, the specific levan yield (Yp/x) which reflects the cell productivity increased with the size increase of the stirred tank bioreactor and reached its maximum value of about 29.4 g/g cells. These results suggested that B. subtilis M could play an important role in levan yield on a large scale in the future. Chemical modifications of B. subtilis M crude levan (CL) into sulfated (SL), phosphorylated (PL), and carboxymethylated levans (CML) were done. The difference in CL structure and its derivatives was detected by FT-IR transmission spectrum. The cytotoxicity of CL and its derivatives were evaluated by HepGII, Mcf-7 and CaCo-2. In general most tested levans forms had no significant cytotoxicity effect. In fact, the carboxymethylated and phosphrylated forms had a lower anti-cancer effect than CL. On the other hand, SL had the highest cytotoxicity showing SL had a significant anti-cancer effect. The results of cytotoxicity and cell viability were statistically analyzed using three-way ANOVA.
Item Type: | Article |
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Uncontrolled Keywords: | Bacillus subtilis, Chemical modification, Cytotoxicity, Fermentor, Levan |
Subjects: | Q Science > QD Chemistry |
Divisions: | Science |
ID Code: | 96944 |
Deposited By: | Widya Wahid |
Deposited On: | 04 Sep 2022 07:26 |
Last Modified: | 04 Sep 2022 07:26 |
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