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High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation

Khairil Anwar, N. A. K. and Hassan, N. and Mohd. Yusof, N. and Idris, A. (2021) High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation. Industrial Crops and Products, 166 . ISSN 0926-6690

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Official URL: http://dx.doi.org/10.1016/j.indcrop.2021.113478

Abstract

Bio-succinic acid is produced from inorganic salt pretreated oil palm empty fruit bunch (OPEFB) for the first time through simultaneous saccharification and fermentation (SSF). OPEFB is an agriculture waste that can be converted into succinic acid (SA) which is a high value added product sought after in the chemical industry. In this study OPEFB is pretreated using sequential inorganic salt (sodium phosphate dodecahydrate and zinc chloride) before undergoing SSF using Actinobacillus succinogenes ATCC 55618. Parameters that influenced the SA production such as substrate loading, inoculum size and enzyme loading are investigated using design of experiments. The results revealed that biomass loading and enzyme loading have significant impact in SA yield. Under condition of 100 g/L biomass, 5% inoculum and cellulase enzyme 40 FPU/g OPEFB, maximum concentration of SA (65.2 g/L) and yield (0.65 g/g OPEFB) are obtained. Subsequently the model developed for predicting SA production has been successfully validated.

Item Type:Article
Uncontrolled Keywords:actinobacillus succinogenes, biomass loading, empty fruit bunch
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:94072
Deposited By: Narimah Nawil
Deposited On:28 Feb 2022 13:17
Last Modified:28 Feb 2022 13:17

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