Isolation and screening of amylolytic yeast from paphiopedilum sp., originating from Bedugul Botanical Garden, Bali, Indonesia

Abstract

Amylase (E.C.3.2.1.1) are the enzyme that works as catalyst in the hydrolysis of starch into simple monomers. Amylase enzymes are widely used in various industrial fields such as textile, food, paper and other industries. Compared to other organisms, yeasts can produce enzymes more effectively and safer for the environment. Amylolytic yeast can be isolated from flower substrates as it contains sugar for the very limited condition of yeast growth. This study aims to isolate, select and characterize amylolytic yeasts on the substrate of Paphiopedilum sp. from Bedugul Botanical Garden, Bali. Yeast isolation was carried out with direct and washing method, followed by screening conducted on YPSA medium with diffusion agar method. Results showed that 19 yeast isolates were obtained with characteristics of 73.9% white-mucoid, 21.05% cream-mucoid and 5.26% light flesh-mucoid. Screening results showed that 10 isolates which coded by P1, P4, P6, P10, P11, P12, P13, P14, P15 and P19, were able to produce amylase enzyme. The potential yeast isolates in yielding amylase with P12 isolate codes had amylolytic index 0,45 mm. from this research, it can be found the symbiosis between yeasts and plants are happening in certain ways.