Universiti Teknologi Malaysia Institutional Repository

Metagenomic data of bacterial community from different land uses at the river basin, Kelantan

Rupert, Rennielyn and Lie, Grace Joy Chin Wei and John, Daisy Vanitha and Annammala, Kogila Vani and Jani, Jaeyres and Rodrigues, Kenneth Francis (2020) Metagenomic data of bacterial community from different land uses at the river basin, Kelantan. Data in Brief, 33 . pp. 1-6. ISSN 2352-3409


Official URL: http://dx.doi.org/10.1016/j.dib.2020.106351


The data provided in the article includes the sequence of bacterial 16S rRNA gene from a high conservation value forest, logged forest, rubber plantation and oil palm plantation collected at Kelantan river basin. The logged forest area was previously notified as a flooding region. The total gDNA of bacterial community was amplified via polymerase chain reaction at V3-V4 regions using a pair of specific universal primer. Amplicons were sequenced on Illumina HiSeq paired-end platform to generate 250 bp paired-end raw reads. Several bioinformatics tools such as FLASH, QIIME and UPARSE were used to process the reads generated for OTU analysis. Meanwhile, R&D software was used to construct the taxonomy tree for all samples. Raw data files are available at the Sequence Read Archive (SRA), NCBI and data information can be found at the BioProject and BioSample, NCBI. The data shows the comparison of bacterial community between the natural forest and different land uses.

Item Type:Article
Uncontrolled Keywords:clustering analysis, kelantan river basin, land-uses, metagenomics, taxonomy tree
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
T Technology > TD Environmental technology. Sanitary engineering
Divisions:Sustainability Research Alliance
ID Code:91712
Deposited By: Yanti Mohd Shah
Deposited On:27 Jul 2021 13:46
Last Modified:27 Jul 2021 13:46

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