Universiti Teknologi Malaysia Institutional Repository

Proximate analysis and bioactivity study on acoustically isolated elaeis guineensis leaves extract

Romes, N. B. and Wahab, R. A. and Hamid, M. A. (2019) Proximate analysis and bioactivity study on acoustically isolated elaeis guineensis leaves extract. In: 2nd International Conference on Biosciences and Medical Engineering 2019: Towards Innovative Research and Cross-Disciplinary Collaborations, ICBME 2019, 11-12 Apr 2019, Bali, Indonesia.

[img]
Preview
PDF
481kB

Official URL: https://dx.doi.org/10.1063/1.5125505

Abstract

Extracts of bioactive compounds from Elaeis guineensis leaves previously acoustically isolated using a probe sonicator that yielded the highest total phenolic content (TPC) was subjected to proximate analysis and antioxidant capacity tests such as ferric reducing antioxidant power assay (FRAP) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) and antibacterial activity. These tests were aimed to gauge the feasibility of incorporating the extract as an active ingredient in a topically applied nanoemulsion. Results revealed that the leaves extract with a TPC of 209.70 mg gallic acid equivalent (GAE)/g) yielded a FRAP value of 1,417.40 μM Fe2+/mg and exhibited strong DPPH radical scavenging capacity with an IC50 value of 9.899 ± 0.050 μg/mL. The value seen here was equivalent to that of positive controls, Trolox and butylated hydroxytoluene (BHT). Consequently, the proximate composition of the leaves was found to comprise of 18.8% moisture content, 5.2% ash, 11.2% protein, 7.1% fat, 57.7% carbohydrate and energy value of 339.5 kcal/100g. E. guineensis leaves extract showed resistance towards the Gram-positive bacteria, Bacillus subtilis at 50 and 100 mg/mL, respectively, but did not inhibit the growth of the Gram-negative bacteria, Escherichia coli, and Pseudomonas aeruginosa.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:elaeis guineensis leaves, total phenolic content, antibacterial activity
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:89997
Deposited By: Narimah Nawil
Deposited On:29 Mar 2021 05:57
Last Modified:29 Mar 2021 05:57

Repository Staff Only: item control page