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Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells

Suhaimi, Siti Hasyimah and Hasham, Rosnani and Idris, Mohamad Khairul Hafiz and Ismail, Hassan Fahmi and Mohd. Ariffin, Nor Hazwani and Abdul Majid, Fadzilah Adibah (2019) Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells. Molecules, 24 (22). p. 4183. ISSN 1420-3049

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Official URL: http://dx.doi.org/10.3390/molecules24224183

Abstract

Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surfacemethodology (RSM)was performed to develop an extractionmethodwithmaximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21min, ultrasound amplitudes 62A, and solvent composition 70%ethanol inwater. The crude extractwas further fractionated using solid-phase extraction (SPE),where six sequential fractions that varied in polarity (0-100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.

Item Type:Article
Uncontrolled Keywords:Prostate cancer, Rosmarinic acid
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:88611
Deposited By: Widya Wahid
Deposited On:15 Dec 2020 18:35
Last Modified:15 Dec 2020 18:35

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