Universiti Teknologi Malaysia Institutional Repository

Development of antibody anti-FimC-Salmonella typhi as a detection kit model of typhoid diseases by antigen capture approach

Nurjayadi, M. and Fera Kurnia Dewi, Fera Kurnia Dewi and Kartika, I. and Hasan, Umar and Setianingsih, Ida and Nur Asiah, Nur Asiah and Wiguna, Delia Ayu and Anis Marcella, Anis Marcella and F. Puspasari, F. Puspasari and Sulfianti, Asri and Agustini, Kurnia and Wardoyo, Wibowo Mangun and El-Enshasy, H. (2019) Development of antibody anti-FimC-Salmonella typhi as a detection kit model of typhoid diseases by antigen capture approach. Biocatalysis and Agricultural Biotechnology, 19 . p. 101157. ISSN 1878-8181

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Official URL: http://dx.doi.org/10.1016/j.bcab.2019.101157


Typhoid fever is a world health problem, with 200,000 recorded death annually in developing countries. The discovery of new drug discovery and detection methods for typhoid is still continuing. In previous research, the 31 kilo Dalton (kDa)recombinant protein Fim-C-S typhi was successfully expressed. It was also reported that the recombinant protein Fim-C-S. typhi could induce the occurrence of antibodies. This study aims to further develop anti-Fim-C-S. typhi antibodies as a detection tool. The sensitivity evaluated by western immunoblotting analysis indicated that anti-Fim-C-S. typhi antibodies can significantly recognize its antigen at a minimum level of 0.125 μg. The specificity evaluation of anti-FimC-S. typhi antibodies against S. typhi bacteria extract protein showed that anti-Fim-C-S. typhi antibodies could recognize S. typhi extract protein at ± 29 KDa and ±60 kDa. In addition, anti-Fim-C-S. typhi antibody did not recognize healthy blood extract proteins. Simulation in healthy blood samples containing bacterial antigen S. typhi and recombinant antigen Fim-C-S. typhi produce bands of 29 kDa, 31 kDa and 60 kDa have been also studied. It can be concluded that anti-Fim-C-S. typhi antibodies can be used in the development of prototype detection tool. The results from this study are expected to provide a foundation to the development of a detection methods for S. typhi that are sensitive, specific, safe and simple.

Item Type:Article
Uncontrolled Keywords:Prototype detection tool, Typhoid diseases
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Medical Engineering
ID Code:87397
Deposited By: Widya Wahid
Deposited On:08 Nov 2020 11:56
Last Modified:08 Nov 2020 11:56

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