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Characterization, optimization and stability studies on Candida rugosa lipase supported on nanocellulose reinforced chitosan prepared from oil palm biomass

Elias, Nursyafiqah and Chandren, Sheela and Abdul Razak, Fazira Ilyana and Jamalis, Joazaizulfazli and Widodo, Nashi and Abdul Wahab, Roswanira (2018) Characterization, optimization and stability studies on Candida rugosa lipase supported on nanocellulose reinforced chitosan prepared from oil palm biomass. International Journal of Biological Macromolecules, 114 . pp. 306-316. ISSN 0141-8130

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Official URL: http://dx.doi.org/10.1016/j.ijbiomac.2018.03.095

Abstract

The contribution of chitosan/nanocellulose (CS-NC) to the enzymatic activity of Candida rugosa lipase covalently bound on the surface of CS-NC (CRL/CS-NC) was investigated. Cellulosic material from oil palm frond leaves (OPFL) were bleached, alkaline treated and acid hydrolyzed to obtain the purified NC and used as nano-fillers in CS. XRD, Raman spectroscopy and optical fluorescence microscopic analyses revealed existence of strong hydrogen bonds between CS and the NC nanofillers. The CRLs were successfully conjugated to the surface of the CS-NC supports via imine bonds that occurred through a Schiff's based mechanism. Process parameters for the immobilization of CRL were assessed for factors temperature, concentration of glutaraldehyde and pH, to afford the highest enzyme activity to achieve maximum conversion of butyl butyrate within 3 h of incubation. Conversion as high as 88% was reached under an optimized condition of 25 °C, 0.3% glutaraldehyde concentration and buffer at pH 7. Thermal stability of CRL/CS-NCs was 1.5-fold greater than that of free CRL, with biocatalysts reusability for up to 8 successive esterification cycles. This research provides a promising approach for expanding the use of NC from OPFL for enhancing enzyme activity in favour of an alternative eco-friendly means to synthesize butyl butyrate.

Item Type:Article
Uncontrolled Keywords:Chitosan, Nanocellulose
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:85801
Deposited By: Widya Wahid
Deposited On:28 Jul 2020 10:45
Last Modified:28 Jul 2020 10:45

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