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Effects of bisphenol a on neonatal cardiomyocytes beating rate and morphology

Rasdi, Zatilfarihiah and Hanafi, Noorul Izzati and Sheikh Abdul Kadir, Siti Hamimah and Ab. Rahim, Sharaniza and Siran, Rosfaiizah and Othman, Mohd. Hafiz Dzarfan and Kamaludin, Roziana and Abdul Hamid Hasani, Narimah and Syed Ahmad Fuad, Syed Baharom (2018) Effects of bisphenol a on neonatal cardiomyocytes beating rate and morphology. Jurnal Teknologi, 80 (6). pp. 141-147. ISSN 0127-9696


Official URL: http://dx.doi.org/10.11113/jt.v80.12286


Bisphenol A (BPA) has been utilised excessively at a global capacity of 2.9 billion kg/year. It is widely used in manufacturing polycarbonate polymers and epoxy resins. Hence, humans are potentially exposed to this chemical substance in their daily life. As a typical endocrine disruptor, BPA exhibits detectable hormone-like properties. Many studies have been linking BPA exposure in humans with the risk of developing cardiovascular disease, however the direct exposure of BPA on cardiomyocytes beating rates and morphology have not been entirely explored. Therefore, in this study, we aimed to investigate the effects of BPA on cells structure and function of neonatal rat cardiomyocytes culture. Cardiomyocytes were isolated from 0 to 2 days old newborn rats and treated with 0.001 to 100 µM concentration of BPA. All cardiomyocytes were subjected to immunostaining, beating frequency assessment assay, MTS assay and Scanning Electron microscopy (SEM). In immunostaining, cardiomyocytes showed positive staining for F-actin. This staining allows identification of the cells thus differentiate cardiomyocytes from other cell types. Significance effects of BPA on cardiomyocytes were observed in MTS assay (p<0.05) and beating rates (p<0.01). Significant reduction (48%-64%, ± 1.5280) was observed in beating rate of cardiomyocytes exposed to 0.1 to 100 µM of BPA. Meanwhile in MTS assay, significant reduction (54%, 0.067 ± 0.0026) in cell viability was observed in cells exposed to 0.1 µM of BPA only. Interestingly, under SEM, cardiomyocytes showed altered cell surface homogeneity after BPA exposure. Exposure of 0.1 to 100 µM BPA lead to flatten of cardiomyocytes cell surface and blurring of the cell borders. This study offers an in vitro evidence of BPA effects on cardiomyocytes morphology and beating rates, thus suggest the potential adverse effect of BPA exposure. However, further investigation would be required to understand how BPA effects normal cells morphology and beating rates of heart cells.

Item Type:Article
Uncontrolled Keywords:beating rates, bisphenol A, cardiomyocytes, cells morphology, cytotoxicity
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:85646
Deposited By: Yanti Mohd Shah
Deposited On:20 Jul 2020 09:24
Last Modified:20 Jul 2020 09:24

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