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A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS

Chua, Lee Suan and Abdul Latiff, Norliza and Ware, Ismail (2018) A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS. Journal of Analytical Chemistry, 73 (2). pp. 167-175. ISSN 1061-9348

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Official URL: http://dx.doi.org/10.1134/S1061934818020065

Abstract

Herbs and herbal based products have been widely used, but some products might contain no herbal ingredient as claimed in the product label. Therefore, it is crucial to develop a fast, sensitive and reliable method to analyze the herbs and their finished products. The roots of Eurycoma longifolia, and the health supplement of E. longifolia and Cordyceps were used as the sample matrices in this study. Sonication assisted extraction was applied to extract the marker compounds, namely eurycomanone and cordycepin from E. longifolia and Cordyceps, respectively. The presence of the marker compounds was established by high throughput ultra-performance liquid chromatography tandem mass spectrometry (UPLC‒MS/MS) using a highly sensitive and selective technique of multiple reaction monitoring (MRM). The positive ion transitions in MRM for eurycomanone and cordycepin are m/z 409 → 391 and m/z 252 → 136, respectively. The test method was validated for its robustness, accuracy, precision, linearity, detection and quantitation limits, as well as estimated for its measurement uncertainty.

Item Type:Article
Uncontrolled Keywords:Cordyceps, Eurycoma longifolia
Subjects:T Technology > TP Chemical technology
Divisions:Chemical and Energy Engineering
ID Code:85624
Deposited By: Widya Wahid
Deposited On:07 Jul 2020 12:59
Last Modified:07 Jul 2020 12:59

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