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Microbiological analysis of drinking water from water vending machines

Muhammad, Mustapha Salisu and Abdul-Wahab, Mohd Firdaus and Saidin, Mohammad Abdul Razis and Asraf, Muhammad Hariz and Nik Malek, Nik Ahmad Nizam (2020) Microbiological analysis of drinking water from water vending machines. Malaysian Journal of Fundamental and Applied Sciences, 16 (2). pp. 186-189. ISSN 2289-5981


Official URL: https://mjfas.utm.my/index.php/mjfas/article/view/...


Commercial water vending machines are gaining popularity nowadays among the general public, probably due to the ease of obtaining clean drinking water. However, improper maintenance of the machines can lead to bacterial contamination. Hence, this study aimed to investigate and determine the microbiological characteristics of drinking water from Water Vending Machines (WVM) by isolating and characterizing culturable bacteria in the water and nozzle swab samples. The samples were obtained from WVM at eight different locations around Johor Bahru, Johor, Malaysia. Several unique bacterial isolates were found, from both Gram-positive and Gram-negative groups. Polymerase chain reaction amplification and 16S rRNA sequence analysis suggested that these isolates are from Pseudomonas, Bacillus, and Stenotrophomonas genera. In situ water quality tests which include pH, conductivity, and total dissolved solids were also conducted. Two samples from the inlet source have pH and conductivity values slightly above the reference values stipulated in drinking water regulations. The findings presented here suggest the importance of regular service maintenance of the WVM to ensure that the water samples meet the standard stipulated by the authority.

Item Type:Article
Uncontrolled Keywords:Bacterial isolation, drinking water, water vending machines, public health
Subjects:T Technology > T Technology (General)
ID Code:85593
Deposited By: Fazli Masari
Deposited On:30 Jun 2020 16:54
Last Modified:30 Jun 2020 16:54

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