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High quality of bacterial dna extraction from Corbicula fluminea (Müller, 1774) tissue in Kelantan

Dee, Koh Han and Appalasamy, Suganthi and Md. Nasir, Siti Nor Aini and Abdullah, Faizuan and Mohamad Nor, Maryana and Rak, Aweng Eh (2020) High quality of bacterial dna extraction from Corbicula fluminea (Müller, 1774) tissue in Kelantan. Malaysian Journal of Fundamental and Applied Sciences, 16 (2). pp. 161-165. ISSN 2289-5981

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Official URL: https://mjfas.utm.my/index.php/mjfas/article/view/...

Abstract

Corbicula fluminea is a freshwater clam that is served as a popular traditional food in Kelantan, Malaysia. However, there are limited studies that report on high quality bacterial metagenome deoxyribonucleic acid (DNA) from C. fluminea. Therefore, this study compares the effectiveness and efficiency of conventional cetyltrimethylammonium bromide (CTAB) protocol, a commercial kit, and modified CTAB protocol for bacterial DNA extraction from the soft tissue surface of raw C. fluminea. The instruments used to examine the quality of the extracted bacterial DNA were DeNovix DS-11 spectrophotometer, gel electrophoresis machine, and UV transilluminator. The results showed that the bacterial DNA extracted from modified CTAB protocol had the highest purity and integrity with the A260/A280 ratio of 1.92 ± 0.01 and A260/A230 ratio of 1.83 ± 0.06 as well as the DNA band with minimum smear. This concludes that modified CTAB protocol is the best approach for the bacterial extraction from the C. fluminea.

Item Type:Article
Uncontrolled Keywords:Corbicula fluminea, high quality DNA, modified CTAB, bacterial metagenome DNA extraction
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:85584
Deposited By: Fazli Masari
Deposited On:30 Jun 2020 16:53
Last Modified:30 Jun 2020 16:53

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