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Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study

Onoja, Emmanuel and Chandren, Sheela and Abdul Razak, Fazira Ilyana and Abdul Wahab, Roswanira (2018) Enzymatic synthesis of butyl butyrate by Candida rugosa lipase supported on magnetized-nanosilica from oil palm leaves: Process optimization, kinetic and thermodynamic study. Journal of the Taiwan Institute of Chemical Engineers, 91 . pp. 105-118. ISSN 1876-1070

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Official URL: http://dx.doi.org/10.1016/j.jtice.2018.05.049

Abstract

The present study reports on Candida rugosa lipase (CRL) supported on magnetized nanosilica from oil palm leaves (CRL/Gl-A-SiO2-MNPs) and its utilization in the catalytic synthesis of butyl butyrate. Statistical optimization of process parameters, viz: enzyme loading, incubation time, temperature and substrate molar ratio (1-butanol:n-butyric acid), was executed using the Box–Behnken Design. Under optimized conditions, using 3.5 mg/mL of CRL/Gl-A-SiO2-MNPs, molar ratio of 2:1, incubated at 45 °C a 93.9% yield of ester was achieved in 3 h. CRL/Gl-A-SiO2-MNPs-catalyzed the esterification reaction according to Bi-Bi Ping Pong mechanism. The obtained kinetic values corresponded to Vmax of 0.298 mM/min, as well as Michaelis-Menten constants for substrates, n-butyric acid (KM a) and 1-butanol (KM b) of 17.274 and 13.780 mM, respectively. The values for Kcat, Keff and inhibition constant for 1-butanol (Ki b) for the CRL/Gl-A-SiO2-MNPs-catalyzed esterification are 5.109/min, 0.371/min mM−1 and 288.184 mM, respectively. CRL/Gl-A-SiO2-MNPs exhibited higher thermal-stability than the aggregated CRL, as seen in the attained higher values of half-life (45.894 min), D-value (152.45 min), Ed (125 KJ/mol), ΔHd∘ (122.64 KJ/mol) and ΔGd∘ (11.957 KJ/mol) at 70 °C. Storage stability of CRL/Gl-A-SiO2 MNPs was improved by ∼ 9 fold over the aggregated CRL when incubated at room temperature.

Item Type:Article
Uncontrolled Keywords:kinetic study, magnetite, nanosilica, oil palm leaves, optimization, thermodynamic
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:85516
Deposited By: Yanti Mohd Shah
Deposited On:30 Jun 2020 16:49
Last Modified:30 Jun 2020 16:49

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