Universiti Teknologi Malaysia Institutional Repository

Biomolecular aspects of second order limit language

Ahmad, Muhammad Azrin and Sarmin, Nor Haniza and Abdul Wahab, Mohd. Firdaus and Heng, Fong Wan and Yusof, Yuhani (2018) Biomolecular aspects of second order limit language. Malaysian Journal of Fundamental and Applied Sciences, 14 (1). pp. 15-19. ISSN 2289-5981


Official URL: http://dx.doi.org/10.11113/mjfas.v14n1.727


The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing system. Recently, second order limit language, a type of the splicing language, has been extensively explored. Before this, several types of splicing languages have been experimentally proven. Therefore, in this paper, a laboratory experiment was conducted to validate the existence of a second order limit language. To accomplish it, an initial strand of double-stranded DNA, amplified from bacteriophage lambda, was generated through polymerase chain reaction to generate thousands of copies of double-stranded DNA molecules. A restriction enzyme and ligase were added to the solution to complete the reaction. The reaction mixture was then subjected to polyacrylamide gel electrophoresis to separate biological macromolecules according to their sizes. A mathematical model derived at the early study was used to predict the approximate length of each string in the splicing language. The results obtained from the experiment are then used to verify the mathematical model of a second order limit language. This study shows that the theory on the second order limit language is biologically proven hence the model has been validated.

Item Type:Article
Uncontrolled Keywords:mathematical modelling, splicing system
Subjects:Q Science > QA Mathematics
ID Code:85500
Deposited By: Widya Wahid
Deposited On:30 Jun 2020 16:46
Last Modified:30 Jun 2020 16:46

Repository Staff Only: item control page