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Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice

Bharudin, Izwan and Abu Bakar, Mohd. Faizal and Hashim, Noor Haza Fazlin and Mat Isa, Mohd. Noor and Alias, Halimah and Raih, Mohd. Firdaus and Md. Illias, Rosli and Najimudin, N. and Mahadi, Nor Muhammad and Abu Bakar, Farah Diba and Abdul Murad, Abdul Munir (2018) Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice. Marine Environmental Research, 137 . pp. 169-176. ISSN 0141-1136

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Official URL: http://dx.doi.org/10.1016/j.marenvres.2018.03.007

Abstract

Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and −12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each library were randomly picked and sequenced. Comparative analyses from the resulting ESTs in each condition identified several groups of genes required for cold adaptation. Additionally, 319 unique transcripts that encoded uncharacterised functions were identified in the −12 °C library and are currently unique to G. antarctica. Gene expression analysis using RT-qPCR revealed two of the unknown genes to be up-regulated at −12 °C compared to 0 °C and 15 °C. These findings further contribute to the collective knowledge into G. antarctica cold adaptation and as a resource for understanding the ecological and physiological tolerance of psychrophilic microbes in general.

Item Type:Article
Uncontrolled Keywords:Antifreeze proteins, Cold adaptation
Subjects:Q Science > Q Science (General)
Divisions:Biosciences and Medical Engineering
ID Code:85018
Deposited By: Widya Wahid
Deposited On:29 Feb 2020 21:19
Last Modified:29 Feb 2020 21:19

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