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Volatolomics combined terahertz time - domain spectral analyses of colon cancer in vitro

Arshad, Ahmad Zulhilmi and Munajat, Yusof and Ghoshal, Sib Krishna and Jamal, Rahman and Johdi, Nor Adzimah and Raja Ibrahim, Raja Kamarulzaman and Zulkhairi, Hanif (2019) Volatolomics combined terahertz time - domain spectral analyses of colon cancer in vitro. Jurnal Teknologi, 81 (5). pp. 105-112. ISSN 2180-3722


Official URL: https://dx.doi.org/10.11113/jt.v81.13310


Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THz-TDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GC-MSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.

Item Type:Article
Uncontrolled Keywords:Volatolomics analysis, colon cancer, Chromatography, Terahertz spectra, gas analysis
Subjects:Q Science > QC Physics
ID Code:84770
Deposited By: Fazli Masari
Deposited On:27 Feb 2020 04:59
Last Modified:27 Feb 2020 04:59

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