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Production of biobutanol by clostridium beijerinki sr1 using sea water medium

Md. Salleh, Madihah and Yahya, Adibah and Ibrahim, Zaharah and Chong, Chun Shiong and Ramanathan, Shankar (2018) Production of biobutanol by clostridium beijerinki sr1 using sea water medium. In: Asean Federation of Biotechnology Malaysia Chapter International Symposium, 19 September 2018 through 21 September 2018, Sarawak.

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Abstract

Biobutanol produced by Clostridium sp. via acetone-butanol-ethanol fermentation (ABE) under strict anaerobic condition served as the most promising biofuel. During ABE fermentation, culture undergoes a metabolic shift from acidogenic phase to solventogenic phase for the formation of solvents. In this preliminary study, production of biobutanol using locally isolated strain using sea water was performed in batch culture. Analysis of filtered sea water from Bakar Batu, Johor showed total carbon and nitrogen of 0.1629% (w/v) and 0.003% (w/v), respectively with C/N ratio of 0.0016. The sodium, calcium, magnesium and potassium concentration were 176.60, 21.92, 23.63 and 58.96 ppm, respectively. The strain used was isolated from contaminated lake sediment in Port Klang, Selangor was further identified as Clostridium beijerinckii SR1 using 16S rRNA studies with accession number of KJ934638. Application of complete Reinforced Clostridium Medium (RCM) with sea water resulted in highest butanol production and productivity of 0.416 g/L and 0.009 g/L/h, respectively with yield of 15.46%. The Yp/s and Yp/x value obtained were 0.15 g/g and 2.10 g/g, respectively. Total cell concentration (X) obtained was 0.23 g/L with µmax and doubling time (td) of 0.090 h-1 and 15.47 h-1, respectively. Application RCM medium without an addition of vitamin solutions (thiamine and biotin) resulted in biobutanol production and productivity of 0.398 g/L and 0.008 g/L/h with yield of 15.48%. Total cell concentration was 0.27 g/L with µmax and doubling time (td) of 0.100 h-1and 14.06 h-1, respectively. Thus, sea water which contains essential minerals in acceptable concentration was successfully utilized for potential biobutanol production in future.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:Sea water, biobutanol
Subjects:Q Science > QR Microbiology
Divisions:Science
ID Code:83913
Deposited By: Widya Wahid
Deposited On:30 Sep 2019 21:55
Last Modified:20 Nov 2019 11:18

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