Probiotic microbe activity for application as anti- fungal and feed intake rate of ruminants

Abstract

In industry Lactobacillus and yeast (Saccharomyces cerevisiae) have been added to animal feed to increase ruminant feed intake. In the current study, we aimed to investigate the symbiotic effect between Lactobacillus rhamnosus (LAB) and yeast on antifungal activity in relation to increase feed intake of mix-bred Jamnapari goats. First, maximum biomass yield of innoculum LAB and S.cerevisiae was determined by using different ratio of initial substrate (molasses) concentration and percent of innoculum. A mixed culture of 4.94% LAB and 4.60% S. cerevisiae with 6.72 g/l molasses resulted in highest biomass yield of cell 3.18 ± 0.25 g/l. The formulation of mix culture was found to produce the highest anti-fungal activity 37.08% ± 2.53 mycelium growth of Aspergillus flavus as compared with single culture of LAB 63.07% ± 0.81 and S. cerevisiae 64.24%. The formulation was used for silage production through solid-state fermentation. Then the effect of silage on ruminant feed intake was studied by comparing ruminant feed of silage S3 (100% silage) and S2 which was 25% silage added with 75% mix feed (50% tapioca leaves + 50% napier grass) with commercial feed (non-silage content) as control parameter such as S5 90% mix feed (50% tapioca leaves + 50% napier grass) added with 10% soy waste, S1 100% mix feed (50% tapioca leaves + 50% napier grass) and S4 99% mix feed (50% tapioca leaves + 50% napier grass) added with 1% urea which applied through four male mix-bred Jamnapari goat. Both feed with silage content were able to increase feed intake activity (100% consumption) as compared with commercial feeds. The result of this study showed that mixed culture LAB and S. cerevisiae could provide advantage to the animal feed industry in term of improving the process of anti-fungal with stimulating appetite in ruminants without any chemical supplement.