Universiti Teknologi Malaysia Institutional Repository

Synthesis of geranyl propionate in a solvent-free medium using Rhizomucor miehei lipase covalently immobilized on chitosan–graphene oxide beads

Isah, A. A. and Mahat, N. A. and Jamalis, J. and Attan, N. and Zakaria, I. I. and Huyop, F. and Wahab, R. A. (2017) Synthesis of geranyl propionate in a solvent-free medium using Rhizomucor miehei lipase covalently immobilized on chitosan–graphene oxide beads. Preparative Biochemistry and Biotechnology, 4 (2). pp. 199-210. ISSN 1082-6068

Full text not available from this repository.

Official URL: http://dx.doi.org/10.1080/10826068.2016.1201681

Abstract

The chemical route of producing geranyl propionate involves the use of toxic chemicals, liberation of unwanted by-products as well as problematic separation process. In view of such problems, the use of Rhizomucor miehei lipase (RML) covalently bound onto activated chitosan–graphene oxide (RML-CS/GO) support is suggested. Following analyses using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and thermogravimetry, properties of the RML-CS/GO were characterized. A response surface methodological approach using a 3-level-four-factor (incubation time, temperature, substrate molar ratio, and stirring rate) Box–Behnken design was used to optimize the experimental conditions to maximize the yield of geranyl propionate. Results revealed that 76 ± 0.02% of recovered protein had yielded 7.2 ± 0.04 mg g−1 and 211 ± 0.3% U g−1 of the maximum protein loading and esterification activity, respectively. The actual yield of geranyl propionate (49.46%) closely agreed with the predicted value (49.97%) under optimum reaction conditions (temperature: 37.67°C, incubation time: 10.20 hr, molar ratio (propionic acid:geraniol): 1:3.28, and stirring rate: 100.70 rpm) and hence, verifying the suitability of this approach. Since the method is performed under mild conditions, the RML-CS/GO biocatalyst may prove to be an environmentally benign alternative for producing satisfactory yield of geranyl propionate.

Item Type:Article
Uncontrolled Keywords:aromatic ester, Box-Behnken design, enzyme
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:80527
Deposited By: Narimah Nawil
Deposited On:22 May 2019 06:50
Last Modified:22 May 2019 06:50

Repository Staff Only: item control page