Effect of continuous therapeutic ultrasound on breast cancer cells

Abstract

The aim of this study is to investigate the potential of continuous therapeutic ultrasound as an alternative therapy to treat breast cancer disease. The study starts with the optimization of the cell seeding numbers, followed by exposure the cells with the ultrasound with two different doses, then MTT assay was used to investigate the cells viability/toxicity. The doses of therapeutic ultrasound were applied on breast cancer cell lines (MCF-7) and normal cell lines (Chinese Hamster Ovary). In this treatment, cells were exposed to the therapeutic ultrasound for three days interval. Based on the MTT assay, it was revealed that exposure of therapeutic ultrasound shows an inhibition effect to MCF-7 cells and less harm on CHO cells. However, when comparing both doses, it shows that dose 2 (3MHz, 0.2W/cm2, 10 minutes, continues cycle, distance from the bottom to the plate is 0.5 cm) have a better effect than dose 1 (3MHz, 0.1W/cm2, 10 minutes, continues cycle, distance from the bottom to the plate is 0.5 cm). These results indicated that continuous therapeutic ultrasound has an effect in inhibiting the breast cancer cells growth and has less harm effect on normal cell.