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Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina

Khalili, Elham and Huyop, Fahrul and Manan, Fatin Myra Abd and Wahab, Roswanira Abdul (2017) Optimization of cultivation conditions in banana wastes for production of extracellular beta-glucosidase by Trichoderma harzianum Rifai efficient for in vitro inhibition of Macrophomina phaseolina. Biotechnology and Biotechnological Equipment, 31 (5). pp. 921-934. ISSN 1310-2818

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Official URL: https://www.tandfonline.com/doi/full/10.1080/13102...

Abstract

The present study was carried out to optimize the physical and nutritional parameters for cultivation of Trichoderma harzianum Rifai for production of extracellular beta-glucosidase, an alternative to chemcial fungicides to control Macrophomina phaseolina, which causes charcoal rot disease in crops. Response surface methodology by the Box-Behnken design (BBD) based on four factors (temperature, carbon sources, inoculum size and pH) was used for optimization of the cultivation conditions to produce beta-glucosidase using agro-industrial banana wastes. The highest -glucosidase activity (1483.27U/mL) was attained under optimized cultivation conditions: approximate to 36 degrees C and pH 7.3, using carbon source and inoculum size of 10% (w/v) and 5% (w/v), respectively. It is noteworthy that the low deviation values (0.09-0.44%) in the verification experiments (R1, R2 and R3) inferred the generated model was accurate to predict optimal cultivation conditions of T. harzianum Rifai. Likewise, the obtained diameters of inhibition zones ranging from 58.00-38.66mm following treatment with beta-glucosidase was found comparable to other in vitro tests using pure T. harzianum isolates and chemical fungicides. Hence, the findings indicated that it was feasible to use beta-glucosidase as a greener alternative to chemical fungicides for control of M. phaseolina infection and consequently, for protection of crops against charcoal rot disease.

Item Type:Article
Uncontrolled Keywords:Trichoderma harzianum T12, beta-glucosidase, response surface methodology, Box-Behnken, green fungicide
Subjects:Q Science > QH Natural history
Divisions:Biosciences and Medical Engineering
ID Code:77526
Deposited By: Widya Wahid
Deposited On:27 Sep 2018 12:14
Last Modified:27 Sep 2018 12:14

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