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Tertiary structure prediction of bromelain from Ananas Comosus using comparative modelling method

Tap, Fatahiya Mohamed and Abd. Majid, Fadzilah Adibah and Khairudin, Nurul Bahiyah Ahmad (2017) Tertiary structure prediction of bromelain from Ananas Comosus using comparative modelling method. International Journal of Advanced and Applied Sciences, 4 (12, 1). pp. 31-35. ISSN 2313-3724

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Abstract

Bromelain is a general name for a family of sulfhydryl which can be found in the proteolytic enzyme group from pineapples (Ananas Comosus). This study focuses on the prediction of three dimensional structures (3D) of stem bromelain using the method of comparative modelling. The amino acid sequence of bromelain was obtained from the NCBI database was used as a tool to search for proteins with known 3D structures related to the target sequence. Suitable template was chosen based on >30% sequence similarity and lowest e-value. Based on these criteria, 1YAL was selected as the best template with 55% of sequence similarity and 9x10(-52) of e-value. (c) 2017 The Authors. Published by IASE.

Item Type:Article
Uncontrolled Keywords:Bromelain, Comparative modelling, Sequence alignment
Subjects:T Technology > TP Chemical technology
Divisions:Chemical Engineering
ID Code:77285
Deposited By: Fazli Masari
Deposited On:30 Jun 2018 05:27
Last Modified:30 Jun 2018 05:27

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