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Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris

Md. Tab, M. and Hashim, N. H. F. and Abu Bakar, F. D. and Illias, R. and Najimudin, N. and Mahadi, N. M. and Murad, A. M. A. (2017) Characterization and potential applications of a recombinant antifreeze protein from an antarctic yeast Glaciozyma antarctica produced in Pichia pastoris. Malaysian Applied Biology, 46 (1). pp. 213-218. ISSN 0126-8643

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Abstract

Ice recrystallization during thawing post-cryopreservation results in extensive cellular damage and ultimately leads to cell death and reduced cell viability. Antifreeze proteins (AFPs) are a group of proteins that allow organisms to survive in subzero environments. These proteins have thermal hysteresis and ice recrystallization inhibitory activities. In this present study, we demonstrated the efficiency of a recombinant antifreeze protein from the Antarctic yeast, Glaciozyma antarctica, as a recrystallization inhibitor (RI) of ice growth and assessed its application as a cryopreservative of the fungal cutinase enzyme against freeze-thaw cycles. Recombinant Afp1 from G. antarctica, a psychrophilic yeast, has been produced in a methylotrophic yeast, Pichia pastoris, system that results in the expression of a hyper-glycoprotein (~55 kDa). Recombinant Afp1 exhibits antifreeze functions: thermal hysteresis (TH) and recrystallization inhibition where the highest TH values recorded for ~0.5°C at 10 mg/mL. The cryoprotective effects of Afp1 on purified recombinant cutinase showed that Afp1 can retain enzymatic activity up to ~20% when subjected to several cycles of freeze thawing. These findings indicate that Afp1 might act as a cryoprotective agent and thus, has great potential in biotechnology applications.

Item Type:Article
Uncontrolled Keywords:Glaciozyma antarctica, Recrystallization inhibition
Subjects:T Technology > TP Chemical technology
Divisions:Chemical Engineering
ID Code:76795
Deposited By: Fazli Masari
Deposited On:30 Apr 2018 22:07
Last Modified:30 Apr 2018 22:07

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