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Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae)

Jibril, S. and Sirat, H. M. and Basar, N. (2017) Bioassay-guided isolation of antioxidants and ?-Glucosidase inhibitors from the root of cassia sieberiana D.C. (fabaceae). Records of Natural Products, 11 (4). pp. 406-410. ISSN 1307-6167

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Abstract

Bioassay-guided isolation was used to investigate the antioxidants and α-glucosidase inhibitors from extract of root of Cassia sieberiana. The ethyl acetate fraction demonstrated strong free radical scavenging (DPPH and ABTS+) and potent α-glucosidase inhibition. The subsequent fractionation and purification of the ethyl acetate fraction using silica gel chromatography and Sephadex LH-20, yielded; islandicin (1), chrysophanol (2), physcion (3), emodin (4), quercetin (5), kaempferol (6), dihydrokaempferol (7), and piceatannol (8). Quercetin (5) showed the most active antioxidant activity with IC50 values of 1.58 mM and 1.30 mM against DPPH and ABTS+ radicals, followed by piceatannol (8) with IC50 values of 3.96 mM and 3.28 mM, which is better than the standard BHT (with IC50 value 8.93 mM) and trolox (with IC50 value 8.25 mM), for DPPH and ABTS+ radicals scavenging activities, respectively. For the α-glucosidase inhibitory assay, quercetin (5) and piceatannol (8) showed higher potency against α-glucosidase with IC50 values of 5.73 μM and 7.37 μM respectively, than standard quercetin with IC50 value of 9.20 μM and acarbose with IC50 value of 14.12 μM. This study presents the first report on the α-glucosidase inhibitors from root of C. sieberiana and all the compounds are isolated from this source for the first time.

Item Type:Article
Uncontrolled Keywords:Antioxidant, Cassia sieberiana, Phytochemicals, ?-glucosidase
Subjects:Q Science > QD Chemistry
Divisions:Science
ID Code:75725
Deposited By: Widya Wahid
Deposited On:30 Apr 2018 13:12
Last Modified:30 Apr 2018 13:12

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