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Electrical impedance spectroscopy for detection of cells in suspensions using microfluidic device with integrated microneedles

Mansor, M. A. and Takeuchi, M. and Nakajima, M. and Hasegawa, Y. and Ahmad, M. R. (2017) Electrical impedance spectroscopy for detection of cells in suspensions using microfluidic device with integrated microneedles. Applied Sciences (Switzerland), 7 (2). ISSN 2076-3417

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

In this study, we introduce novel method of flow cytometry for cell detection based on impedance measurements. The state of the art method for impedance flow cytometry detection utilizes an embedded electrode in the microfluidic to perform measurement of electrical impedance of the presence of cells at the sensing area. Nonetheless, this method requires an expensive and complicated electrode fabrication process. Furthermore, reuse of the fabricated electrode also requires an intensive and tedious cleaning process. Due to that, we present a microfluidic device with integrated microneedles. The two microneedles are placed at the half height of the microchannel for cell detection and electrical measurement. A commercially-available Tungsten needle was utilized for the microneedles. The microneedles are easily removed from the disposable PDMS (Polydimethylsiloxane) microchannel and can be reused with a simple cleaning process, such as washing by ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting passing cells at the sensing area. Therefore, this device is suitable for low-cost medical and food safety screening and testing process in developing countries.

Item Type:Article
Uncontrolled Keywords:Cell concentration detection, Impedance flow cytometry, Microneedle
Subjects:Q Science > QH Natural history
Divisions:Biosciences and Medical Engineering
ID Code:75346
Deposited By: Widya Wahid
Deposited On:22 Mar 2018 19:03
Last Modified:22 Mar 2018 19:03

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