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Selective and low potential electrocatalytic oxidation and sensing of l-cysteine using metal impurity containing carbon black modified electrode

Sornambikai, S. and Abdul Kadir, M. R. and Kumar, A. S. and Ponpandian, N. and Viswanathan, C. (2017) Selective and low potential electrocatalytic oxidation and sensing of l-cysteine using metal impurity containing carbon black modified electrode. Analytical Methods, 9 (48). pp. 6791-6800. ISSN 1759-9660

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Abstract

The detection of thiol-containing amino acids, particularly l-cysteine (l-CySH), without any interference from other biochemicals is a challenging research interest in electroanalytical chemistry. Amongst various electrodes, the nanogold modified electrode has been reported to be effective for low potential electrochemical oxidation (∼0 V vs. Ag/AgCl) and sensing of l-CySH. Herein, we report a conductive carbon black (CB, CL-08) modified glassy carbon electrode (GCE/CL-08) with a high surface area (1000 m2 g-1), prepared by a simple drop-casting technique for efficient electrocatalytic oxidation at the lowest oxidation potential, -0.13 V vs. Ag/AgCl, and sensing of l-CySH in a pH 6.65 phosphate buffer solution. The trace metal impurities such as Ni (0.18 wt%) and Fe (0.42 wt%) in CL-08 are found to be key for such unique and unusual electrocatalytic property observed in this study. Compared with the multiwalled carbon nanotube and graphite powder modified electrodes, the GCE/CL-08 showed about 400 mV reduction in the oxidation potential and twenty times enhancement in the current signal for the CySH. A chronoamperometry detection of l-CySH on the GCE/CL-08 at an applied potential = -0.13 V vs. Ag/AgCl yielded a current linearity from 50 to 700 μM with a LOD = 45.87 nM. There are no interferences by common biochemicals such as ascorbic acid, dopamine, uric acid, xanthine, hypoxanthine and homo-cysteine on the l-CySH oxidation potential. Selective chronoamperometric detection of l-CySH in the blood serum demonstrated ∼100% recovery value as a validation for the present protocol.

Item Type:Article
Uncontrolled Keywords:Carbon nanotubes, Chronoamperometry
Subjects:Q Science > QD Chemistry
Divisions:Biosciences and Medical Engineering
ID Code:75307
Deposited By: Fazli Masari
Deposited On:27 Mar 2018 06:11
Last Modified:27 Mar 2018 06:11

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