Sornambikai, S. and Abdul Kadir, M. R. and Kumar, A. S. and Ponpandian, N. and Viswanathan, C. (2017) Selective and low potential electrocatalytic oxidation and sensing of l-cysteine using metal impurity containing carbon black modified electrode. Analytical Methods, 9 (48). pp. 6791-6800. ISSN 1759-9660
Full text not available from this repository.
Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....
Abstract
The detection of thiol-containing amino acids, particularly l-cysteine (l-CySH), without any interference from other biochemicals is a challenging research interest in electroanalytical chemistry. Amongst various electrodes, the nanogold modified electrode has been reported to be effective for low potential electrochemical oxidation (∼0 V vs. Ag/AgCl) and sensing of l-CySH. Herein, we report a conductive carbon black (CB, CL-08) modified glassy carbon electrode (GCE/CL-08) with a high surface area (1000 m2 g-1), prepared by a simple drop-casting technique for efficient electrocatalytic oxidation at the lowest oxidation potential, -0.13 V vs. Ag/AgCl, and sensing of l-CySH in a pH 6.65 phosphate buffer solution. The trace metal impurities such as Ni (0.18 wt%) and Fe (0.42 wt%) in CL-08 are found to be key for such unique and unusual electrocatalytic property observed in this study. Compared with the multiwalled carbon nanotube and graphite powder modified electrodes, the GCE/CL-08 showed about 400 mV reduction in the oxidation potential and twenty times enhancement in the current signal for the CySH. A chronoamperometry detection of l-CySH on the GCE/CL-08 at an applied potential = -0.13 V vs. Ag/AgCl yielded a current linearity from 50 to 700 μM with a LOD = 45.87 nM. There are no interferences by common biochemicals such as ascorbic acid, dopamine, uric acid, xanthine, hypoxanthine and homo-cysteine on the l-CySH oxidation potential. Selective chronoamperometric detection of l-CySH in the blood serum demonstrated ∼100% recovery value as a validation for the present protocol.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Carbon nanotubes, Chronoamperometry |
Subjects: | Q Science > QD Chemistry |
Divisions: | Biosciences and Medical Engineering |
ID Code: | 75307 |
Deposited By: | Fazli Masari |
Deposited On: | 27 Mar 2018 06:11 |
Last Modified: | 27 Mar 2018 06:11 |
Repository Staff Only: item control page