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Cervicare™ induces apoptosis in HeLa and CaSki cells through ROS production and loss of mitochondrial membrane potential

Amini, Neda and Abdul Majid, Fadzilah Adibah and Marvibaigi, Mohsen and Supriyanto, Eko and Jaganathan, Saravana Kumar and Tet Soon, Wong Tet and Nasiri, Rozita and Hamzehalipour, Javad (2016) Cervicare™ induces apoptosis in HeLa and CaSki cells through ROS production and loss of mitochondrial membrane potential. RSC Advances, 6 (29). pp. 24391-24417. ISSN 2046-2069

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Cervicare™ is a poly-herbal preparation comprised of a combination of 6 plants; most have demonstrated antimicrobial and anticancer properties in preclinical studies. The effect of the ethanol and aqueous extracts of Cervicare™ on cell proliferation and apoptosis using cervical cancer HeLa and CaSki cells was investigated for the first time in the present study. MTT assay results showed that Cervicare™ extracts exerted time- and dose-dependent inhibition of cell viability. The hallmark properties of apoptosis like cell shrinkage and cytoplasmic condensation were observed using an inverted phase contrast microscope, ethidium bromide/acridine orange and Hoechst 33342/propidium iodide fluorescent staining methods. Furthermore, our results demonstrated that Cervicare™ extracts induced apoptosis in HeLa and CaSki cells by ROS generation and mitochondrial depolarization in a concentration dependent manner. The results showed that Cervicare™ extracts were capable of suppressing cell migration and inhibiting colony formation in a dose-dependent manner. Moreover, western blot analysis demonstrated the involvement of a mitochondria-dependent apoptosis pathway in the apoptosis inducing activity of Cervicare™ ethanol extract in HeLa cells. GC-MS analysis of the ethanolic extract afforded the identification of 40 substances, showing that it was primarily composed of anti-cancerous compounds such as xanthorrhizol (60.40%), octacosane (9.93%) and squalene (1.24%). Together, these results point out the Cervicare™ mediated inhibition of HeLa cell growth via induction of apoptosis and that it may be a potential anticancer agent which deserves further investigation.

Item Type:Article
Uncontrolled Keywords:Cell death, Cell membranes, Cell proliferation, Derivatives, Drug products, Electrophoresis, Ethanol, Mitochondria, Apoptosis-inducing activity, Concentration-dependent manners, Ethanol and aqueous extracts, Mitochondrial membrane potential, Phase contrast microscopes, Potential anticancer agents, Proliferation and apoptosis, Western-blot analysis, Cytology
Subjects:Q Science > QH Natural history
Divisions:Biosciences and Medical Engineering
ID Code:74244
Deposited By: Siti Nor Hashidah Zakaria
Deposited On:28 Nov 2017 15:42
Last Modified:28 Nov 2017 15:42

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