Cheng, Shu Ian and Stuckey, David C. (2016) Protein separation mechanisms in surfactant precipitation systems. Separation Science and Technology (Philadelphia), 51 (1). pp. 181-191. ISSN 0149-6395
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Abstract
Separation of globular proteins (cytochrome c and ribonuclease A) from buffer using precipitation by sodium bis-(2-ethylhexyl) sulfosuccinate (AOT), and their recovery with a counterionic surfactant, trioctylmethylammonium chloride (TOMAC) was investigated. The molar ratio between AOT and the protein (R) required for complete removal was 17 for cytochrome c, and 22 for ribonuclease A. Finally, three mixtures of the two proteins and lysozyme (studied earlier by us) were used to determine the factors controlling separation: selectivity was a strong function of surface charge distribution, indicating that charge interactions between the surfactant and surface groups of different proteins was driving precipitation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Proteins, Separation, Surface active agents, Surface charge, Charge interactions, Globular proteins, Precipitation systems, Protein recovery, Protein separations, Sodium bis(2-ethylhexyl)sulfosuccinate, Three mixtures, Trioctylmethylammonium chlorides, Precipitation (chemical) |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 73980 |
| Deposited By: | Fahmi Moksen |
| Deposited On: | 23 Nov 2017 06:19 |
| Last Modified: | 23 Nov 2017 06:19 |
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