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Synthesis and characterization of collagen-hydroxyapatite immobilized on polydopamine grafted stainless steel

Tapsir, Zafirah and Saidin, Syafiqah (2016) Synthesis and characterization of collagen-hydroxyapatite immobilized on polydopamine grafted stainless steel. Surface and Coatings Technology, 285 . pp. 11-16. ISSN 0257-8972

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Abstract

Hydroxyapatite (HA) and collagen have been coated on metallic implants to accelerate osseointegration. Most methods to coat HA require high sintering temperature, high cost and high energy power while the methods to coat collagen commonly produce unstable coating. Therefore, in this study, a polydopamine film was used as an intermediate layer to immobilize HA and collagen type I on a medical grade stainless steel (SS316L) implant to overcome those disadvantages. The SS316L disks were pre-treated and grafted with a polydopamine film. It was then covalently immobilized with collagen fibers at different immersion times (6, 12 and 24. h). The disks were further biomineralized with HA in simulated body fluid (SBF) for 7. days. The film surfaces were characterized by FTIR, FESEM-EDX, XRD and contact angle analyses to investigate the chemical composition, morphology, crystallinity and wettability properties. The collagen and carbonated HA (lath-like surface) were successfully immobilized on the polydopamine film with less agglomeration as the immersion time in the collagen solution increased. Increasing the immersion time accelerated the activation of carboxylic groups in the collagen to form an amide cross-linkage for heterogenous nucleation of HA. Furthermore, the crystallinity and wettability properties were also enhanced with the closest theoretical Ca/P ratio. As a conclusion, the immobilization of collagen at 24. h has produced better HA formation and wettability property that might be beneficial for the attachment and proliferation of osteoblast cells on biomedical implants.

Item Type:Article
Uncontrolled Keywords:Chemical analysis, Collagen, Grafting (chemical), Hydroxyapatite, Sintering, Wetting, Chemical compositions, Contact angle analysis, Heterogenous nucleation, High sintering temperatures, Intermediate layers, Polydopamine, Simulated body fluids, Synthesis and characterizations, Stainless steel
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Medical Engineering
ID Code:73968
Deposited By: Fahmi Mokhsen
Deposited On:23 Nov 2017 12:17
Last Modified:23 Nov 2017 12:17

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