Almaki, Javad Hamzehalipour and Nasiri, Rozita and Idris, Ani and Abdul Majid, Fadzilah Adibah and Salouti, Mojtaba and Wong, Tet Soon and Dabagh, Shadab and Marvibaigi, Mohsen and Amini, Neda (2016) Synthesis, characterization and in vitro evaluation of exquisite targeting SPIONs-PEG-HER in HER2+ human breast cancer cells. Nanotechnology, 27 (10). ISSN 0957-4484
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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....
Abstract
A stable, biocompatible and exquisite SPIONs-PEG-HER targeting complex was developed. Initially synthesized superparamagnetic iron oxide nanoparticles (SPIONs) were silanized using 3-aminopropyltrimethoxysilane (APS) as the coupling agent in order to allow the covalent bonding of polyethylene glycol (PEG) to the SPIONs to improve the biocompatibility of the SPIONs. SPIONs-PEG were then conjugated with herceptin (HER) to permit the SPIONs-PEG-HER to target the specific receptors expressed over the surface of the HER2+ metastatic breast cancer cells. Each preparation step was physico-chemically analyzed and characterized by a number of analytical methods including AAS, FTIR spectroscopy, XRD, FESEM, TEM, DLS and VSM. The biocompatibility of SPIONs-PEG-HER was evaluated in vitro on HSF-1184 (human skin fibroblast cells), SK-BR-3 (human breast cancer cells, HER+), MDA-MB-231 (human breast cancer cells, HER-) and MDA-MB-468 (human breast cancer cells, HER-) cell lines by performing MTT and trypan blue assays. The hemolysis analysis results of the SPIONs-PEG-HER and SPIONs-PEG did not indicate any sign of lysis while in contact with erythrocytes. Additionally, there were no morphological changes seen in RBCs after incubation with SPIONs-PEG-HER and SPIONs-PEG under a light microscope. The qualitative and quantitative in vitro targeting studies confirmed the high level of SPION-PEG-HER binding to SK-BR-3 (HER2+ metastatic breast cancer cells). Thus, the results reflected that the SPIONs-PEG-HER can be chosen as a favorable biomaterial for biomedical applications, chiefly magnetic hyperthermia, in the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Biocompatibility, Blood, Cell culture, Coupling agents, Cytology, Diseases, Fourier transform infrared spectroscopy, Iron compounds, Iron oxides, Medical applications, Metal nanoparticles, Nanoparticles, Polyethylene glycols, Polyethylenes, Superparamagnetism, Synthesis (chemical), 3-aminopropyl trimethoxysilane, Biomedical applications, Breast Cancer, Herceptin, Human breast cancer cells, Iron oxide nanoparticle, Superparamagnetic iron oxide nanoparticles, Superparamagnetics, Cells, epidermal growth factor receptor 2, ERBB2 protein, human, ferric ion, ferric oxide, macrogol derivative, magnetite nanoparticle, breast tumor, cell survival, chemistry, female, hemolysis, human, in vitro study, materials testing, metabolism, pathology, photon correlation spectroscopy, physiology, synthesis, tumor cell line, Breast Neoplasms, Cell Line, Tumor, Cell Survival, Dynamic Light Scattering, Female, Ferric Compounds, Hemolysis, Humans, In Vitro Techniques, Magnetite Nanoparticles, Materials Testing, Polyethylene Glycols, Receptor, ErbB-2 |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Chemical Engineering |
| ID Code: | 73869 |
| Deposited By: | Fahmi Moksen |
| Deposited On: | 20 Nov 2017 08:14 |
| Last Modified: | 20 Nov 2017 08:14 |
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