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On-demand guided bone regeneration with microbial protection of ornamented SPU scaffold with bismuth-doped single crystalline hydroxyapatite: augmentation and cartilage formation

Selvakumar, M. and Srivastava, P. and Pawar, H. S. and Francis, N. K. and Das, B. and Sathishkumar, G. and Subramanian, B. and Jaganathan, S. K. and George, G. and Anandhan, S. and Dhara, S. and Nando, G. B. and Chattopadhyay, S. (2016) On-demand guided bone regeneration with microbial protection of ornamented SPU scaffold with bismuth-doped single crystalline hydroxyapatite: augmentation and cartilage formation. ACS Applied Materials and Interfaces, 8 (6). pp. 4086-4100. ISSN 1944-8244

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Guided bone regeneration (GBR) scaffolds are futile in many clinical applications due to infection problems. In this work, we fabricated GBR with an anti-infective scaffold by ornamenting 2D single crystalline bismuth-doped nanohydroxyapatite (Bi-nHA) rods onto segmented polyurethane (SPU). Bi-nHA with high aspect ratio was prepared without any templates. Subsequently, it was introduced into an unprecedented synthesized SPU matrix based on dual soft segments (PCL-b-PDMS) of poly(ε-caprolactone) (PCL) and poly(dimethylsiloxane) (PDMS), by an in situ technique followed by electrospinning to fabricate scaffolds. For comparison, undoped pristine nHA rods were also ornamented into it. The enzymatic ring-opening polymerization technique was adapted to synthesize soft segments of PCL-b-PDMS copolymers of SPU. Structure elucidation of the synthesized polymers is done by nuclear magnetic resonance spectroscopy. Sparingly, Bi-nHA ornamented scaffolds exhibit tremendous improvement (155%) in the mechanical properties with excellent antimicrobial activity against various human pathogens. After confirmation of high osteoconductivity, improved biodegradation, and excellent biocompatibility against osteoblast cells (in vitro), the scaffolds were implanted in rabbits by subcutaneous and intraosseous (tibial) sites. Various histological sections reveal the signatures of early cartilage formation, endochondral ossification, and rapid bone healing at 4 weeks of the critical defects filled with ornamented scaffold compared to SPU scaffold. This implies osteogenic potential and ability to provide an adequate biomimetic microenvironment for mineralization for GBR of the scaffolds. Organ toxicity studies further confirm that no tissue architecture abnormalities were observed in hepatic, cardiac, and renal tissue sections. This finding manifests the feasibility of fabricating a mechanically adequate nanofibrous SPU scaffold by a biomimetic strategy and the advantages of Bi-nHA ornamentation in promoting osteoblast phenotype progression with microbial protection (on-demand) for GBR applications.

Item Type:Article
Uncontrolled Keywords:Aspect ratio, Biocompatibility, Biodegradation, Biomechanics, Biomimetics, Bismuth, Bone, Cartilage, Crystalline materials, Histology, Magnetic resonance spectroscopy, Microchannels, Nuclear magnetic resonance spectroscopy, Polyurethanes, Ring opening polymerization, Tissue, Electrospun scaffolds, Endochondral ossification, Enzymatic ring-opening polymerization, Guided bone regeneration, Nano-hydroxyapatite, Poly (epsiloncaprolactone), Segmented polyurethanes, Single-crystalline bismuth, Scaffolds (biology), antiinfective agent, baysilon, bismuth, dimeticone, hydroxyapatite, polycaprolactone, polyester, animal, Bacterial Infections, bone regeneration, chemistry, chondrogenesis, drug effects, human, male, rabbits and hares, tissue scaffold, Animals, Anti-Infective Agents, Bacterial Infections, Bismuth, Bone Regeneration, Chondrogenesis, Dimethylpolysiloxanes, Durapatite, Humans, Male, Polyesters, Rabbits, Tissue Scaffolds
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Medical Engineering
ID Code:73860
Deposited By: Haliza Zainal
Deposited On:20 Nov 2017 02:21
Last Modified:20 Nov 2017 02:21

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