Universiti Teknologi Malaysia Institutional Repository

Identification of novel bacterial species capable of degrading dalapon using 16S rRNA sequencing

Hamzehalipour Almaki, J. and Nasiri, R. and Tet Soon, W. and Huyop, F. Z. (2016) Identification of novel bacterial species capable of degrading dalapon using 16S rRNA sequencing. Jurnal Teknologi, 78 (6). pp. 77-82. ISSN 0127-9696

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Abstract

2,2-dichloropropionic acid (2,2DCP) is used as herbicide in agricultural industry and it is one of the halogenated organic compounds distributed widely in the world causing contamination. In this study, a bacterial strain isolated from contaminated soil where halogenated pesticides applied in Universiti Teknologi Malaysia and it was named “JHA1”. Bacterium JHA1 was able to utilize 2,2 dichloropropionate 2,2-DCP or (Dalapon) as a source of carbon and energy. Based on 16S rRNA analysis, the isolate showed 87% identity to Terrabacter terrae strain PPLB. The identity score was lower than 98% so that it was suggested to be new organisms that worth for further investigations if it will be proven that this is novel. Therefore, current isolate was designated as Terrabacter terrae JHA1. The isolate grew in the minimal media containing 10 mM, 15 mM, 20 mM and 25 mM of 2,2- DCP as the sole energy and carbon source and the best growth rate was in 20 mM as the optimum concentration of 2,2-DCP while bacterial growth was inhibited in medium with 30 mM 2,2-DCP.

Item Type:Article
Uncontrolled Keywords:16S rRNA, dalapon, identification
Subjects:T Technology > TP Chemical technology
Divisions:Chemical Engineering
ID Code:72447
Deposited By: Narimah Nawil
Deposited On:22 Nov 2017 12:07
Last Modified:22 Nov 2017 12:07

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