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Characterization and antibacterial activity of chlorhexidine loaded silver-kaolinite

Jou, S. K. and Malek, N. A. N. N. (2016) Characterization and antibacterial activity of chlorhexidine loaded silver-kaolinite. Applied Clay Science, 127-12 . pp. 1-9. ISSN 0169-1317

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Chlorhexidine acetate-loaded silver-kaolinite (CA-Ag-Kaol) was prepared and characterised, and its application as an antibacterial agent was studied. CA-Ag-Kaol was prepared by the adsorption of chlorhexidine acetate (CA) (0.5 mmol/L) on Ag (50% of the Cation Exchange Capacity (CEC) of kaolinite) on kaolinite. Kaolinite (Kaol), silver-kaolinite (Ag-Kaol), CA-modified kaolinite (CA-Kaol) and CA-Ag-Kaol were characterised by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, field-emission scanning-electron microscopy (FESEM), energy dispersive X-ray (EDX) spectroscopy, zeta potential analysis and dispersion behavior measurements. The modification of kaolinite with cationic silver and chlorhexidine ions did not change the structure of kaolinite, and the characterization of the kaolinite samples revealed the successful loading of cationic silver and chlorhexidine ions on the kaolinite. The antibacterial assay of the samples was carried out against Escherichia coli ATCC 11229, Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212 using the disc diffusion technique (DDT) and the minimum inhibition concentration (MIC) technique. Based on the antibacterial assay, CA-Ag-Kaol showed better antibacterial activity than Ag-Kaol and CA-Kaol, and it performed well in both distilled water and a 0.9% saline solution. Gram-positive bacteria were more susceptible to the antibacterial behavior of Ca-Ag-Kaol than Gram-negative bacteria. In conclusion, silver-kaolinite that has been loaded with chlorhexidine acetate can be used as an effective antibacterial agent because of its high antibacterial activity against wide spectrum of bacteria in solutions containing electrolytes (saline solution).

Item Type:Article
Uncontrolled Keywords:Bacteria, Escherichia coli, Field emission microscopes, Fourier transform infrared spectroscopy, Saline water, Scanning electron microscopy, Silver, X ray diffraction, Anti-bacterial activity, Anti-bacterial behaviors, Cation exchange capacities, Chlorhexidine acetate, Field emission scanning electron microscopy, Gram-positive bacterium, Minimum inhibition concentrations, Zeta potential analysis, Kaolinite, acetate, adsorption, concentration (composition), electrolyte, inhibition, kaolinite, microbial activity, silver, Enterococcus faecalis, Escherichia coli, Negibacteria, Posibacteria, Pseudomonas aeruginosa, Staphylococcus aureus
Subjects:R Medicine > R Medicine (General)
Divisions:Biosciences and Medical Engineering
ID Code:72346
Deposited By: Narimah Nawil
Deposited On:20 Nov 2017 08:23
Last Modified:20 Nov 2017 08:23

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