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Isolation and characterization of polyhydroxyalkanoates (PHAS) producing bacteria from brackish stream

Zain, N. A. M. and Zargoun, L. M. A. and Elias, N. F. and Wahab, M. F. A. and Suhaimi, M. S. (2016) Isolation and characterization of polyhydroxyalkanoates (PHAS) producing bacteria from brackish stream. Jurnal Teknologi, 78 (7). pp. 75-81. ISSN 0127-9696


Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....


Polyhydroxyalkanoates (PHAs) are biopolymers which have similar characteristics with petrochemical plastics but a step better due to its biodegradable property. A total of 23 strains were isolated from two different brackish sources. In order to detect the PHAs granules, the PHAs producing bacteria were first screened with Sudan Black B staining. Twenty strains were observed with lipid granules and were subjected to further confirmation with Nile blue staining. From the Nile blue staining, only 10 strains have the ability in producing PHAs and 2 were identified as strong PHAs producers. This study focuses on the 2 strains named S1 and L1. Further identification procedure was carried out and found that strain S1 and L1 belongs to Pseudomonas sp. L1 strain was found to be promising for PHAs production since it accumulated PHAs for about 88.3%. The PHAs produced by this strain was analyzed using Fourier Transform Infrared Spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) analysis and was identified as poly-3-hydroxybutyrate (P-3HB).

Item Type:Article
Uncontrolled Keywords:biopolymer, FTIR, NMR
Subjects:R Medicine > R Medicine (General)
Divisions:Biosciences and Medical Engineering
ID Code:72333
Deposited By: Narimah Nawil
Deposited On:20 Nov 2017 08:23
Last Modified:20 Nov 2017 08:23

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