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A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid

Mohamed, E. and Rahiman, F. A. and Wahab, R. A. and Zain, C. R. C. M. and Javed, M. A. and Huyop, F. (2016) A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid. Journal of Animal and Plant Sciences, 26 (4). pp. 1133-1139. ISSN 1018-7081

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Abstract

A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCA-resistant transformants were selected in tissue cultures containing 60 µg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 g/L MCA compared with 2.0 g/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.

Item Type:Article
Uncontrolled Keywords:Herbicide resistant, Monochloroacetic acid, Tobacco
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Medical Engineering
ID Code:72227
Deposited By: Fazli Masari
Deposited On:22 Nov 2017 20:07
Last Modified:22 Nov 2017 20:07

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