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Systematic analysis of protein interaction network associated with azoospermia

Sabetian, S. and Shamsir, M. S. (2016) Systematic analysis of protein interaction network associated with azoospermia. International Journal of Molecular Sciences, 17 (11). ISSN 1661-6596


Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....


Non-obstructive azoospermia is a severe infertility factor. Currently, the etiology of this condition remains elusive with several possible molecular pathway disruptions identified in the post-meiotic spermatozoa. In the presented study, in order to identify all possible candidate genes associated with azoospermia and to map their relationship, we present the first protein-protein interaction network related to azoospermia and analyze the complex effects of the related genes systematically. Using Online Mendelian Inheritance in Man, the Human Protein Reference Database and Cytoscape, we created a novel network consisting of 209 protein nodes and 737 interactions. Mathematical analysis identified three proteins, ar, dazap2, and esr1, as hub nodes and a bottleneck protein within the network. We also identified new candidate genes, CREBBP and BCAR1, which may play a role in azoospermia. The gene ontology analysis suggests a genetic link between azoospermia and liver disease. The KEGG analysis also showed 45 statistically important pathways with 31 proteins associated with colorectal, pancreatic, chronic myeloid leukemia and prostate cancer. Two new genes and associated diseases are promising for further experimental validation.

Item Type:Article
Uncontrolled Keywords:androgen receptor, beta catenin, Crk associated substrate protein, cyclic AMP responsive element binding protein binding protein, estrogen receptor alpha, phosphoprotein, BCAR1 protein, human, CREBBP protein, human, Crk associated substrate protein, cyclic AMP responsive element binding protein binding protein, DAZAP2 protein, human, RNA binding protein, Article, azoospermia, cell survival, embryo development, gene ontology, germ line, human, mathematical analysis, morphogenesis, protein binding, protein database, protein localization, protein protein interaction, spermatogonium, algorithm, azoospermia, biological model, gene expression regulation, gene regulatory network, genetic database, genetics, male, metabolism, procedures, protein analysis, protein protein interaction, Algorithms, Azoospermia, CREB-Binding Protein, Crk-Associated Substrate Protein, Databases, Genetic, Gene Expression Regulation, Gene Ontology, Gene Regulatory Networks, Humans, Male, Models, Genetic, Protein Interaction Mapping, Protein Interaction Maps, Receptors, Androgen, RNA-Binding Proteins
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Biosciences and Medical Engineering
ID Code:71948
Deposited By: Fazli Masari
Deposited On:20 Nov 2017 08:18
Last Modified:20 Nov 2017 08:18

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