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Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy

Chong, Kah Chun and Thang, Lee Yien and Joselito, P. Quirino and See, Hong Heng (2017) Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi- stacking strategy. Journal of Chromatography A, 1485 . pp. 142-146. ISSN 0021-9673

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Official URL: http://dx.doi.org/10.1016/j.chroma.2017.01.012

Abstract

A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C4D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C4D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2 μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%–99.2%.

Item Type:Article
Additional Information:RADIS System Ref No:PB/2017/11482
Uncontrolled Keywords:microchip electrophoresis, vancomycin
Subjects:Q Science
Divisions:Science
ID Code:66124
Deposited By: Widya Wahid
Deposited On:11 Jul 2017 15:41
Last Modified:11 Jul 2017 15:41

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