Aemi Syazwani, Abdul Keyon and Guijt, Rosanne M. and Bolch, Christopher J. S. and Breadmore, Michael Charles (2014) Transient isotachophoresis-capillary zone electrophoresis with contactless conductivity and ultraviolet detection for the analysis of paralytic shellfish toxins in mussel samples. Journal of Chromatography A, 1364 . p. 302. ISSN 0021-9673
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Official URL: http://dx.doi.org/10.1016/j.chroma.2014.08.074
Abstract
The accumulation of paralytic shellfish toxins (PSTs) in contaminated shellfish is a serious health risk making early detection important to improve shellfish safety and biotoxin management. Capillary electrophoresis (CE) has been proven as a high resolution separation technique compatible with miniaturization, making it an attractive choice in the development of portable instrumentation for early, on-site detection of PSTs. In this work, capillary zone electrophoresis (CZE) with capacitively coupled contactless conductivity detector (C4D) and UV detection were examined with counter-flow transient isotachophoresis (tITP) to improve the sensitivity and deal with the high conductivity sample matrix. The high sodium concentration in the sample was used as the leading ion while l-alanine was used as the terminating electrolyte (TE) and background electrolyte (BGE) in which the toxins were separated. Careful optimization of the injected sample volume and duration of the counter-flow resulted in limit of detections (LODs) ranging from 74.2 to 1020ng/mL for tITP-CZE-C4D and 141 to 461ng/mL for tITP-CZE-UV, an 8-97 fold reduction compared to conventional CZE. The LODs were adequate for the analysis of PSTs in shellfish samples close to the regulatory limit. Intra-day and inter-day repeatability values (percentage relative standard deviation, n=3) of tITP-CZE-C4D and tITP-CZE-UV methods for both migration time and peak height were in the range of 0.82-11% and 0.76-10%, respectively. The developed method was applied to the analysis of a contaminated mussel sample and validated against an Association of Official Analytical Chemists (AOAC)-approved method for PSTs analysis by high performance liquid chromatography (HPLC) with fluorescence detection (FLD) after pre-column oxidation of the sample. The method presented has potential for incorporation in to field-deployable devices for the early detection of PSTs on-site.
Item Type: | Article |
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Uncontrolled Keywords: | paralytic shellfish toxins, transient isotachophoresis |
Subjects: | Q Science |
Divisions: | Science |
ID Code: | 63114 |
Deposited By: | Siti Nor Hashidah Zakaria |
Deposited On: | 15 Jun 2017 01:37 |
Last Modified: | 15 Jun 2017 01:37 |
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