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Separation of N-nitrosamines by micellar electrokinetic chromatography

Md. Ali, Nur Amira and Sanagi, Mohd. Marsin and Wan Ibrahim, Wan Aini (2014) Separation of N-nitrosamines by micellar electrokinetic chromatography. Malaysian Journal of Analytical Sciences, 18 (3). pp. 534-543. ISSN 1394-2506

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Official URL: http://www.ukm.my/mjas/v18_n3/Nur%20Amira_18_3_7.p...

Abstract

A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for separation of three selected N-Nitrosamines namely N-nitrosodipropylamine (NDPA), N-nitrosodibutylamine (NDBA) and N-nitrosodiphenylamine (NDPhA). The effects of composition of the buffer and its pH, concentration of surfactants on the separation and migration times of nitrosamines were investigated. The instrumental variables affecting sensitivity and resolution such as power supply and injection mode were carefully optimized. The best separation was achieved using 40 mM sodium dodecyl sulfate (SDS) as a surfactant in 10 mM phosphate buffer (pH 8.0) at a temperature of 25 ºC, applied voltage of 29 kV, wavelength of 230 nm and electrokinetic injection of 9 s at 5 kV within 10 min analysis time. Excellent linearity was obtained in the concentration range of 2 to 100 μg/mL with coefficients of determination, r2 ≥ 0.979. This method showed good reproducibility with relative standard deviation (RSDs) value ranging from 2.46% to 6.61%. The limits of detection (LOD) and limits of quantification (LOQ) ranged from 0.16 to 0.43 μg/mL and 0.54 to 1.44 μg/mL respectively.

Item Type:Article
Uncontrolled Keywords:surfactants, capillary electrophoresis
Subjects:Q Science
Divisions:Science
ID Code:62559
Deposited By: Widya Wahid
Deposited On:18 Jun 2017 14:29
Last Modified:18 Jun 2017 14:29

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